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ASCP ASCP-MLT Exam Syllabus Topics:

SectionWeightObjectives
Chemistry20–25%- Carbohydrates, lipids, and enzymes
- Electrolytes, acid-base, and toxicology
Hematology20–25%- Laboratory testing and hemostasis
- Physiology and disease states
Laboratory Operations5–10%- Instrumentation and laboratory mathematics
- Quality assessment and safety
Immunology5–10%- Serologic procedures and immune diseases
- Principles of immunology
Blood Banking15–20%- Blood products and components
- Blood group systems and immunology
- Transfusion practice and testing
Urinalysis and Other Body Fluids5–10%- Microscopic analysis and disease correlation
- Physical and chemical testing
Microbiology15–20%- Bacteriology and mycology procedures
- Virology and parasitology testing

ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Sample Questions:

Question 1

The correct answer which best fits the characteristics in this question is Mycobacterium kansasii.
The remaining Mycobacterium strains can be elimated as:
Mycobacterium marinum is considered a fast-grower.
Mycobacterium scrofulaceum produce deep-yellow to orange pigmented colonies when grown in the either the light or dark.
Mycobacterium avium grows colonies which are nonpigmented in the light and dark which do not intensify after light exposure.
What is the MOST likely identification of an acid-fast bacillus that demonstrates the following characteristics?
slow growth
cream to tan colored colonies when grown in the dark
development of yellow pigment upon exposure to light

A. Mycobacterium scrofulaceum
B. Mycobacterium marinum
C. Mycobacterium avium
D. Mycobacterium kansasii


Question 2

The Westgard multi-rule 22S describes the scenario where two consecutive data points fall outside +2SD or
-2SD. If this occurs, then the run must be rejected. This situation is most likely caused by a systematic error.
Which of the following describes the Westgard multi-rule 22S?

A. Two points are within 2SD
B. One control data point falls outside +2s and a second point falls outside - 2s
C. Two control data points are within ± 2s
D. Two consecutive data points fall outside +2SD or -2SD
E. Two consecutive data points fall inside +2s


Question 3

Basophilic stippling is the term used to describe red blood cells that contain tiny particles of RNA within their cytoplasm. Basophilic stippling is associated with many conditions, but is strongly associated with lead poisoning.
Multiple small, dark blue particles scattered throughout the cytoplasm of erythrocytes is/are called:

A. Basophilic stippling
B. Heinz bodies
C. Pappenheimer bodies
D. Howell-Jolly bodies


Question 4

The indole (modified Kovacs' reagent) is used to assess the ability of certain bacteria to produce indole by the deamination of tryptophan. Indole reacts with aldehyde in the Kovac's reagent to produce a red color. An alcoholic layer holds the red color as a ring at the top of the tube used for the idole test.
If a bacteria produces the enzyme tryptophanase to break down the amino acid tryptophan, which of the following tests will be positive with Kovac's reagent?

A. catalase test
B. esculin test
C. citrate test
D. indole test
E. oxidase test


Question 5

Though automated extraction machines have many benefits over manual methods the costs are high and generally require a high throughput of samples in order to justify the costs.
Automated extraction has many benefits over the traditional manual methods. The most important benefit is that the nucleic acid isolated is constantly consistent. There is a reduced amount of manipulation with dramatically decreases the chance of cross contamination. Also, automated extraction machines are considered moderate complexity and can be performed by a wider variety of laboratory professionals.
All of the following are considered benefits of automated isolation and extraction equipment EXCEPT:

A. Automated isolation equipment is cheaper and more practical
B. Automated isolation equipment provides consistent results
C. Automated isolation equipment is considered moderate complexity
D. Automated isolation equipment requires less manipulation


Solutions:

Question 1
Answer: D
Question 2
Answer: D
Question 3
Answer: C
Question 4
Answer: D
Question 5
Answer: A

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